Original scientific paper

Chronic administration of phytoestrogen “daidzein” to ameliorate mean arterial pressure and vascular function in N-G-nitro-L-arginine methyl ester hypertensive rats.

Shahid Prawez ; Department of Pharmacology and Toxicology, Faculty of Veterinary and Animal Sciences, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, India
Azad A. Ahanger ; Sher-e-Kashmir University of Agricultural Sciences and Technology-Kashmir, Shuhama, Alustang, Srinagar, India
Thakur U. Singh ; Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar (UP), India
Santosh K. Mishra ; Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar (UP), India
Souvendra N. Sarkar ; Division of Pharmacology and Toxicology, Indian Veterinary Research Institute, Izatnagar (UP), India
Shafiqur Rahman ; Division of Pathology, Sher-e-Kashmir University of Agricultural Sciences and Technology-Jammu, FVSc&AH, RS Pura, Jammu, J&K, India

Abstract

The ‘silent killer’, hypertension, leads to heart disease, stroke, kidney failure and premature death. The phytoestrogen daidzein has been associated with vaso-protective action similar to oestrogen, with minimal side effects. To explore the vaso-protective activity of daidzein and also its effect on mean arterial pressure (MAP), daidzein was chronically administered in N-G-nitro-L-arginine methyl ester (L-NAME)-hypertensive male Wistar rats for 6 weeks. The male Wistar rats were divided into three groups, namely group-A (control), group-B (L-NAME-treated) and group-C (L-NAME+daidzein treated). After completion of 42 days (6 weeks) of daidzein treatment, MAP and vascular activity were observed in all the groups. Daidzein treatment of L-NAME hypertensive rats (group-C) for 6 weeks significantly decreased the MAP (144 mm Hg) as compared to untreated-L-NAME-hypertensive rats/group-B (173.2 mm Hg), indicating the blood-pressure lowering property of daidzein. Also daidzein significantly increased acetylcholine-induced maximal relaxations of the thoracic aorta isolated from daidzein-treated (Emax = 72.55 %) in comparison to untreated-L-NAME-hypertensive rats (Emax = 39.33 %). The results of the present study suggest that chronic administration of daidzein (0.5 mg/kg/day, s.c.) helps to lower blood pressure, as indicated by a decrease in MAP, and also shows vaso-protective action, as indicated by the improvement in ACh-induced relaxation.

Keywords

hypertension; daidzein; blood pressure; vascular function; rats

Hrčak ID:

145313

URI

https://hrcak.srce.hr/145313

Publication date:

2.9.2015.

Article data in other languages: croatian

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