Short communication, Note
The effects of 2,3,7,8-tetrachloro-dibenzo-p-dioxin on human polymorphonuclear leucocytes
M. Carević
; Croatian National Electricity , Zagreb, Croatia
O. Carević
; Scientific Council for Traffic, Croatian Academy of Science and Arts, Zagreb, Croatia
Abstract
Suspensions of human polymorphonuclear leucocytes were treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) at concentrations 10-8, 10-7,10-6 and 10-5 M. The TCDD provoked a progressive extracellular release of the lysosomal enzyme beta-N-acetylglucosaminidase and the cytoplasmic enzyme lactate dehydrogenase in a dose-dependent fashion. At all concentrations TCDD was much more effective in releasing beta-N-acetylglucosaminidase than lactate dehydrogenas. Different responses of the two enzymes to TCDD might be explained by differential structural affinities of beta-N-acetylglucosaminidase for the lysosomal membrane and of lactate dehydrogenase for the cytoplasm. It is likely that TCDD affected the solubility of beta-N-acetylglucosaminidase to a higher extent than that of lactate dehydrogenase, which is probably firmly attached to the cytoplasm.
Keywords
cytoplasmic enzyme; extracellular enzyme release; lysosomal enzyme
Hrčak ID:
146968
URI
Publication date:
6.10.1994.
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