Short communication, Note

Xenobiotics and biochemical parameters of isolated rat hepatocytes

M. Popović ; Institut za hemiju, Prirodno-matematički fakultet, Novi Sad, Srbija
K. Đaković-Švajcer ; Zavod za farmakologiju i toksikologiju, Medicinski fakultet Novi Sad, Srbija
J. Nerudova ; Institut za higijenu i epidemiologiju, Prag, CSR

Abstract

The authors investigated the effects of the inductors (griseofulvin and fenitoin) and the inhibitors of the cytochrome P-450 (metronidazole, chloramphenicol and phenylbutazone), the effects of the drugs with the purine structure (aminophylline, xanthinol-nicotmate, pentoxiphylline, azothioprine, thioguanine, 6-mercaptopurine), and the effects of several pesticides (lmdane, binapicrile, paratbion) on some biochemical parameters of the rat liver. The following parameters were determined: viability of hepatocytes, the content of glutathione and cytochrome P-450, and the activity of xanthinoxidase and hpoperoxidase. The experiments were performed in vivo and in vitro. The results showed that the cell viability as well as the other parameters studied, were most drastically affected by chloramphenicol, azathioprine and parathion, whereas the other substances elicited less intensive changes.

Keywords

cytochrome P-450; glutathione; xanthinoxidase; lipoperoxidase; pesticides; drug effects; hepatocyte viability

Hrčak ID:

147905

URI

https://hrcak.srce.hr/147905

Publication date:

9.6.1992.

Article data in other languages: serbian

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