Agriculture, Vol. 21 No. 2, 2015.
Professional paper
https://doi.org/10.18047/poljo.21.2.11
DEVELOPMENT OF NEW SEQUENCING TECHNOLOGIES AND THEIR APPLICATION IN GENOME ANALYSIS OF DOMESTIC ANIMALS
Kristina Gvozdanović
; J.J.Strossmayer University of Osijek, Faculty of Agriculture in Osijek, Kralja Petra Svačića 1d, 31000 Osijek, Croatia
Vice Čuljak
; Ministarstvo poljoprivrede, Uprava za veterinarstvo i sigurnost hrane, Veterinarski ured Osijek, Trg Lava Mirskog 3, 31000 Osijek, Croatia
Polonca Margeta
; J.J.Strossmayer University of Osijek, Faculty of Agriculture in Osijek, K. P. Svačića 1d, 31000 Osijek, Croatia
Abstract
Sequencing and detailed study of the genom of domestic animals began in the middle of the last century. It was primarily referred to development of the first generation sequencing methods, i.e. Sanger sequencing method. Next generation sequencing methods are currently the most common methods in the analysis of domestic animals genom. The application of these methods gave us up to 100 time more data in comparison with Sanger method. Analyses including RNA sequencing, genotyping of whole genome, immunoprecipitation associated with DNA microarrays, detection ofmutations and inherited diseases, sequencing ofthemitochondrial genome and many others have been conducted with development and application of new sequencing methods since 2005 until today. Application of new sequencing methods in the analysis ofdomestic animal genome provides better understanding of the genetic basis for important production traits which could help in improving the livestock production.
Keywords
sequencing; Sanger sequencing method; next generation sequencing; domestic animal genome analysis
Hrčak ID:
150621
URI
Publication date:
18.12.2015.
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