Original scientific paper
https://doi.org/10.3325/cmj.2015.56.246
Synthetic vs natural scaffolds for human limbal stem cells
Mirna Tominac Trcin
; University Hospital Centre Sestre Milosrdnice, Tissue bank at University Department of Traumatology, Zagreb, Croatia
Iva Dekaris
; Specialty Eye Hospital Svjetlost, Zagreb and Department of Ophthalmology Medical Faculty, University of Rijeka, Rijeka, Croatia
Budimir Mijović
; Department of Basic Natural and Technical Sciences, Faculty of Textile Technology, University of Zagreb, Zagreb, Croatia
Marina Bujić
; University Hospital Centre Sestre Milosrdnice, Tissue bank at University Department of Traumatology, Zagreb, Croatia
Emilija Zdraveva
; Department of Basic Natural and Technical Sciences, Faculty of Textile Technology, University of Zagreb, Zagreb, Croatia
Tamara Dolenec
; University Hospital Centre Sestre Milosrdnice, Tissue bank at University Department of Traumatology, Zagreb, Croatia
Maja Pauk-Gulić
; Specialty Eye Hospital Svjetlost, Zagreb and Department of Ophthalmology Medical Faculty, University of Rijeka, Rijeka, Croatia
Dragan Primorac
; Eberly College of Science, The Pennsylvania State University, University Park, PA, USA
Josip Crnjac
; University Department for Forensic Sciences, University of Split, Split, Croatia
Branimira Špoljarić
; Faculty of Veterinary Medicine University of Zagreb, Zagreb, Croatia
Gordan Mršić
; Forensic Science Centre “Ivan Vučetić”,Zagreb, Croatia
Krunoslav Kuna
; University Hospital Centre Sestre Milosrdnice, Gynecology and Obstetrics Department, Zagreb, Croatia
Daniel Špoljarić
; Faculty of Veterinary Medicine University of Zagreb, Zagreb, Croatia
Maja Popović
; Faculty of Veterinary Medicine University of Zagreb, Zagreb, Croatia
Abstract
Aim To investigate the impact of synthetic electrospun
polyurethane (PU) and polycaprolactone (PCL) nanoscaffolds,
before and after hydrolytic surface modification, on
viability and differentiation of cultured human eye epithelial
cells, in comparison with natural scaffolds: fibrin and
human amniotic membrane.
Methods Human placenta was taken at elective cesarean
delivery. Fibrin scaffolds were prepared from commercial
fibrin glue kits. Nanoscaffolds were fabricated by electrospinning.
Limbal cells were isolated from surpluses of
human cadaveric cornea and seeded on feeder 3T3 cells.
The scaffolds used for viability testing and immunofluorescence
analysis were amniotic membrane, fibrin, PU, and
PCL nanoscaffolds, with or without prior NaOH treatment.
Results Scanning electron microscope photographs of all
tested scaffolds showed good colony spreading of seeded
limbal cells. There was a significant difference in viability
performance between cells with highest viability cultured
on tissue culture plastic and cells cultured on all other scaffolds.
On the other hand, electrospun PU, PCL, and electrospun
PCL treated with NaOH had more than 80% of limbal
cells positive for stem cell marker p63 compared to only
27%of p63 positive cells on fibrin.
Conclusion Natural scaffolds, fibrin and amniotic membrane,
showed better cell viability than electrospun scaffolds.
On the contrary, high percentages of p63 positive
cells obtained on these scaffolds still makes them good
candidates for efficient delivery systems for therapeutic
purposes
Keywords
Hrčak ID:
151286
URI
Publication date:
15.6.2015.
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