Original scientific paper
https://doi.org/10.3325/cmj.2015.56.326
Oxidative stress impact on growth hormone secretion in the eye
Borna Šarić
; Ophthalmology Clinic, University Hospital Sveti Duh, Zagreb, Croatia
Vlatka Brzović Šarić
; Ophthalmology Clinic, University Hospital Sveti Duh, Zagreb, Croatia
Monika Barberić
; Institute for Medical Laboratory Diagnostics, University Hospital Sveti Duh, Zagreb, Croatia
Jurica Predović
; Ophthalmology Clinic, University Hospital Sveti Duh, Zagreb, Croatia
Vlatko Rumenjak
; Institute for Medical Laboratory Diagnostics, University Hospital Sveti Duh, Zagreb, Croatia
Branimir Cerovski
; University Hospital Center Zagreb, Zagreb, Croatia
Abstract
Aim To evaluate the influence of oxidative stress on extrapituitary
growth hormone (GH) secretion in the eye and to
analyze the interdependence between eye and serum GH
levels under normal and hypoxic conditions.
Methods Pars plana vitrectomy (PPV) was performed in
32 patients with developed proliferative diabetic retinopathy
(PDR) and 49 non-diabetic controls, both of whom
required this procedure as part of their regular treatment
in the period from April 2013 to December 2014. During
PPV, vitreous samples were taken and blood was simultaneously
collected from the cubital vein. GH levels in serum
and vitreous samples were measured by electrochemical
luminescence assay. Oxidative stress was measured by enzyme-
linked immunosorbent assay of advanced oxidation
protein products (AOPP) and lipid hydroperoxide (LPO) in
serum and vitreous.
Results Serum AOPP levels were significantly higher than
vitreous levels in both groups (P < 0.001 for each group)
and LPO levels were significantly higher only in PDR
group (P < 0.001). There was a significant positive correlation
between serum and vitreous LPO levels in PDR group
(r = 0.909; P < 0.001). Serum GH levels were significantly
higher than vitreous levels in both groups (P < 0.001 for
each group). Serum GH levels were significantly higher in
PDR group than in controls (P = 0.012). Vitreous GH values
were slightly higher in PDR group, but the difference was
not significant.
Conclusion Our study confirms that GH production in the
eye is autonomous and independent of oxidative stress or
pituitary GH influence.
Keywords
Hrčak ID:
151539
URI
Publication date:
15.8.2015.
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