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Veterinary Archives, Vol. 86 No. 1, 2016.

Original scientific paper

A nanoparticle-assisted PCR assay for the detection of encephalomyocarditis virus.

Wanzhe Yuan ; College of Animal Medicine, Agricultural University of Hebei, Baoding, Hebei, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding, Hebei, China; North China Research Center of Animal Epidemic Pathogen Biology, Agri
Yanan Li ; College of Animal Medicine, Agricultural University of Hebei, Baoding, Hebei, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding, Hebei, China; North China Research Center of Animal Epidemic Pathogen Biology, Agri
Jianchang Wang ; Center of Technology, Hebei Entry-Exit Inspection and Quarantine Bureau, Shijiazhuang, Hebei, China
Jinfeng Wang ; Center of Technology, Hebei Entry-Exit Inspection and Quarantine Bureau, Shijiazhuang, Hebei, China
Jiguo Sun ; College of Animal Medicine, Agricultural University of Hebei, Baoding, Hebei, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding, Hebei, China; North China Research Center of Animal Epidemic Pathogen Biology, Agri

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Abstract

The encephalomyocarditis virus (EMCV) can cause acute myocarditis in young pigs or reproductive failure in sows. Diseases caused by EMCV currently affect the swine industry worldwide. In this study, a nano polymerase chain reaction (nanoPCR) assay, targeting the 3D gene of EMCV, was developed and its sensitivities and specificities were investigated. The results indicated that the nanoPCR assay is highly sensitive and able to detect 1.2×102 copies/μL of EMCV RNA, as no cross-reaction was observed with other viruses. This is the first report to demonstrate the application of a nanoPCR assay for the detection of EMCV. The sensitive, and specific nanoPCR assay developed in this study can be applied widely in clinical diagnosis and field surveillance of EMCV-infection.

Keywords

encephalomyocarditis virus; nanoPCR; RT-PCR; detection

Hrčak ID:

153488

URI

https://hrcak.srce.hr/153488

Publication date:

15.2.2016.

Article data in other languages: croatian

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