Original scientific paper
https://doi.org/10.5599/jese.232
The interactions between lipase and pyridinium ligands investigated by electrochemical and spectrophotometric methods
Simona Patriche
; Department of Chemistry, Physics and Environment, Faculty of Sciences and Environment, “Dunărea de Jos” University of Galati, 111 Domneasca Street, 800201 Galati, Romania
Elena Georgiana Lupu
; Department of Chemistry, Physics and Environment, Faculty of Sciences and Environment, “Dunărea de Jos” University of Galati, 111 Domneasca Street, 800201 Galati, Romania
Andreea Cârâc
; Department of Fundamental Science, Faculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy of Bucharest, 6 Train Vuia Street, 020956 Bucharest, Romania
Rodica Mihaela Dinică
; Department of Chemistry, Physics and Environment, Faculty of Sciences and Environment, “Dunărea de Jos” University of Galati, 111 Domneasca Street, 800201 Galati, Romania
Geta Cârâc
; Department of Chemistry, Physics and Environment, Faculty of Sciences and Environment, “Dunărea de Jos” University of Galati, 111 Domneasca Street, 800201 Galati, Romania
Abstract
The interaction between pyridinium ligands derived from 4,4’-bipyridine (N,N’-bis(p-bro-mophenacyl)-4,4’-bipyridinium dibromide – Lr) and (N,N’-bis(p-bromophenacyl)-1,2-bis (4-pyridyl) ethane dibromide – Lm) with lipase enzyme was evaluated. The stability of the pyridinium ligands, having an essential role in biological systems, in 0.1 M KNO3 as supporting electrolyte is influenced by the lipase concentration added. The pH and conductometry measurements in aqueous solution suggest a rapid ionic exchange process. The behavior of pyridinium ligands in the presence of lipase is investigated by cyclic voltammetry and UV/Vis spectroscopy, which indicated bindings and changes from the interaction between them. The voltammograms recorded on the glassy carbon elec-trode showed a more intense electronic transfer for the Lr interaction with lipase com-pared to Lm, which is due to the absence of mobile ethylene groups from Lr structure.
Keywords
Enzyme; Pyridine; Cyclic voltammetry; Morphology; Physicochemical properties
Hrčak ID:
156450
URI
Publication date:
21.4.2016.
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