Acta Pharmaceutica, Vol. 66 No. 3, 2016.
Original scientific paper
https://doi.org/10.1515/acph-2016-0033
Olive leaf extract activity against Candida albicans and C. dubliniensis – the in vitro viability study
NATAŠA ZORIĆ
; Agency for Medicinal Products and Medical Devices of Croatia (HALMED), Zagreb, Croatia
NEVENKA KOPJAR
; Institute for Medical Research and Occupational Health, Zagreb, Croatia
KLARA KRALJIĆ
; University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, Croatia
NADA ORŠOLIĆ
; University of Zagreb, Faculty of Science Division of Biology, Zagreb, Croatia
SINIŠA TOMIĆ
; Agency for Medicinal Products and Medical Devices of Croatia (HALMED), Zagreb, Croatia
IVAN KOSALEC
; University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, Croatia
Abstract
Olive leaf extract is characterized by a high content of polyphenols (oleuropein, hydroxytyrosol and their derivatives), which is associated with its therapeutic properties. The objective of the present research was to evaluate the antifungal activity of olive leaf aqueous extract against Candida albicans ATCC 10231 and C. dubliniensis CBS 7987 strains. Minimum inhibitory concentration (MIC) of the extract was determined by several in vitro assays. The extract showed a concentration dependent effects on the viability of C. albicans with MIC value of 46.875 mg mL–1 and C. dubliniensis with MIC value 62.5 mg mL–1.
Most sensitive methods for testing the antifungal effect of the extract were the trypan blue exclusion method and fluorescent dye exclusion method while MIC could not be determined by the method according to the EUCAST recommendation suggesting that herbal preparations contain compounds that may interfere with this susceptibility testing. The fluorescent dye exclusion method was also used for the assessment of morphological changes in the nuclei of the treated cells. According to the obtained results, olive leaf extract is less effective against the tested strains than hydroxytyrosol, an olive plant constituent tested in our previous study.
Keywords
antifungal activity; Candida albicans; Candida dubliniensis; olive leaf extract
Hrčak ID:
156871
URI
Publication date:
30.9.2016.
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