Original scientific paper

Spectrophotometric determination of toxogonin in urine and blood serum

N. Burger ; Zavod za kemiju i biokemiju, Medicinski fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska
Z. Smerić ; Zavod za kemiju i biokemiju, Medicinski fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska
V. Hankonyi ; Zavod za kemiju i biokemiju, Medicinski fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska

Abstract

Water-soluble, coloured complexes formed in the reactions of Toxogonin with either sodium aminepentacyanoferrate(II) (AmPF) or sodium nitro-sylpentacyanoferrate (II) (NP) were used for spectrophotometric determinations ot Toxogonin in urine and blood serum. Toxogonin in urine can be determined with both reagents in the concentration range from 100 to 1 OOO µmol/dm3 of urine (if analysis is performed with 0.1 cm3 of native urine). The coefficient of variation ranges from 2.66 to 5.31% in the determination with AmPF and from 1.92 to 3.75% in the determination with NP. Toxogonin in serum can be determined only by using the NP reagent. In the concentration range from 50 to 500 µmol/dm3 of serum, the coefficient of variation is between 1.98 and 2.24%.

Keywords

Hrčak ID:

157512

URI

https://hrcak.srce.hr/157512

Publication date:

10.10.1984.

Article data in other languages: croatian

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