Original scientific paper

Hydroxycotinine metabolism in rabbit liver, lung and kidney tissue homogenates

M.A. Rashid Mahmud ; Faculty of Pharmacy, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
A. Nikolin ; Faculty of Pharmacy, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
B. Nikolin ; Faculty of Pharmacy, University of Sarajevo, Sarajevo, Bosnia and Herzegovina
M. Lekić ; Faculty of Pharmacy, University of Sarajevo, Sarajevo, Bosnia and Herzegovina

Abstract

Hydroxycotinine metabolism has been investigated in rabbit liver, lung and kidney tissue homogenates, using NADP and NADPH. With NADPH, the liver was the most active organ in metabolizing hydroxycotinine yielding hydroxycotinine-N-oxide (16.3%) as the main metabolic product, along with only little cotinine. With NADP, the formation of cotinine was slightly higher in the kidney and lung tissue incubates than the corresponding formation of cotinine with NADPH. The qualitative identification of the substrate and the metabolic products was carried out by thin-layer chromatography, followed by their quantitative estimation by gas-liquid chromatography.

Keywords

Hrčak ID:

157518

URI

https://hrcak.srce.hr/157518

Publication date:

10.10.1984.

Article data in other languages: croatian

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