Original scientific paper
Knock-down of ELMO1 in Paediatric Rhabdomyosarcoma Cells by Nanoparticle Mediated siRNA Delivery
Xinyue Huang
; Department of Engineering Sciences, University of Oxford, UK
Helen Townley
; Department of Engineering Sciences, University of Oxford, UK; Department of Obstetrics and Gynaecology, University of Oxford, UK
Abstract
Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma that is found in children and has a poor outcome for those with metastatic disease. Two histological groups have been distinguished - embryonal (ERMS) and alveolar (ARMS) forms. The ARMS subtype has higher rates of metastasis, as well as higher levels of ELMO1, which is thought to be involved in cell migration. Therefore, the knock-down of ELMO1 by targeted siRNA could provide a mechanism to prevent the metastatic behaviour of ARMS cells. However, challenges still lie in the delivery of nucleotides to a tumour site. Herein, we have described the use of a variety of mesoporous silica nanoparticles as a delivery system for siRNA that is specific for ELMO1 and shown the effective reduction in cell invasive behaviour in these cells.
Keywords
Cancer; Metastasis; ELMO1; Nanoparticles; siRNA
Hrčak ID:
157699
URI
Publication date:
1.1.2016.
Visits: 773 *