Original scientific paper
The response of mammalian cells in the plateau phase to treatment with manganese
Y. Škreb
; Institute for Medical Research and Occupational Health, Zagreb, Croatia
Abstract
The toxicity of manganese chloride was evaluated for V79 Chinese hamster cells in asynchronous culture during two different periods of their life: the period of exponential growth and the plateau phase. The samples of control and manganese-treated cells were microscopically examined and their proliferation rate was estimated. The inhibition of the cells' colony forming ability served for assessing the toxic potential of three concentrations of manganese chloride during different exposure times. After treatment the cells were seeded at low density. Seven days later the colonies formed by the surviving cells were stained by Giemsa solution, counted and expressed as percentages of the controls considered as hundred per cent. The reduced colony forming ability exhibited by the plateau phase cells proves that they are more susceptible to manganese than the cells growing exponentially, particularly to manganese concentrations above 5 x 10-5 mol and in exposures over 10 hours. The difference in susceptibility to manganese of proliferative and plateau phase V79 Chinese hamster cells is similar to that shown by the same phases in response to various chemicals and drugs such as bleomycine and nitrosuorea derivatives.
Keywords
Hrčak ID:
158841
URI
Publication date:
5.4.1982.
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