Conference paper

Survival of Rat Embryonic Parts after Ectopic Transplantation

Floriana Bulić-Jakuš
Gordana Jurić-Lekić
Maja Vlahović
Ana Katušić
Sandra Marinović-Kulišić
Ljiljana Šerman
Davor Ježek

Abstract

Investigation of the developmental potential of embryonic cells is important in designing novel approaches in the field of regenerative medicine. The purpose of our experiments was to compare the survival of different embryonic tissues transplanted at an ectopic site in vivo. Embryo proper (9.5-day-old), neural retinas (20-day-old), lensectomized eyes (14- and 18-day-old), epiglottis (17-day-old), mandible (13- and 14-day-old), lacrimal gland (17- and 20-day-old) were microsurgically isolated from rat embryos and transplanted under the renal capsule. The embryo-proper survived in transplants for at least 60 days. Fetal rat retina survived in transplants for 180 days forming rosettes. Neural and glia cells with abundant neuropil as well as plexiform layers were found by electron microscopy. The lensectomized eye survived in transplants for 66 days. Transdifferentiation of the retina to lens cells was discovered. The epiglottis, mandible and lacrimal gland survived in transplants for at least 14 days. In the mandible, fully structured teeth developed. This study showed not only the early postimplantation embryo but also various more developed tissues and organs to be able to survive under the renal capsule for at least 14 days. This ectopic site has therefore proved to be a very convenient environment for transplantation experiments.

Keywords

Embryo; Transplantation; Renal capsule; Survival; Differentiation

Hrčak ID:

15397

URI

https://hrcak.srce.hr/15397

Publication date:

1.6.2005.

Article data in other languages: croatian

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