Original scientific paper

The Mutagenic Potential of Chloroform, Orange Oil, Eucalyptus Oil and Halothane by Salmonella/Microsome Assay

Zoran Karlović
Silvana Jukić
Ivana Miletić
Smiljana Britvić
Maja Osmak
Ivica Anić

Abstract

The aim of this study was to examine mutagenic activity of four commercially available gutta-percha solvents by means of the Salmonella/ microsome assay. The examined solvents were: chloroform, orange oil, eucalyptus oil and halothane in amounts of 10 μl, 30 μl, 50 μl, 100 μl and 200 μl. Standard plate incorporation Ames test was preformed by using two tester strains of Salmonella typhimurium, TA 98 and TA 100, with metabolic activation of S9. The results showed toxicity of eucalyptus oil in all aliquots, orange oil in aliquots of 50 μl and above and chloroform in aliquots of 100 μl and 200 μl, but all four substances responded negative to the Ames test. These results indicate that the tested solvents do not possess mutagenic activity toward the Salmonella strains used.

Keywords

Ames test; Chloroform; Orange oil; Eucalyptus oil; Halothane

Hrčak ID:

1377

URI

https://hrcak.srce.hr/1377

Publication date:

15.3.2004.

Article data in other languages: croatian

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