Original scientific paper
https://doi.org/10.20302/NC.2016.25.8
Molecular data do not confirm the Grecian anomalous blue Polyommatus (Agrodiaetus) aroaniensis (Brown, 1976) as a member of the Croatian fauna
Leona Lovrenčić
; Croatian Natural History Museum, Demetrova 1, 10000 Zagreb, Croatia
Martina Podnar
; Croatian Natural History Museum, Demetrova 1, 10000 Zagreb, Croatia
Martina Šašić
; Croatian Natural History Museum, Demetrova 1, 10000 Zagreb, Croatia
Toni Koren
; Croatian Herpetological Society - Hyla, Lipovac I, 7, 10000 Zagreb, Croatia
Nikola Tvrtković
; Alagovićeva 21, 10000 Zagreb, Croatia
Abstract
The presence of the Grecian anomalous blue, Polyommatus (Agrodiaetus) aroaniensis (Brown, 1976) in Croatia has been recently published based on external morphology of collected specimens. The aim of this study was to evaluate the credibility of these findings by using DNA barcoding approach as the species exhibit phenotypic variability and can easily be misidentified with morphologically similar species Polyommatus (Agrodiaetus) ripartii. Therefore, we performed a molecular analysis of the mitochondrial gene for cytochrome c oxidase subunit I (COI) of the samples previously published as P. aroaniensis from different localities in Croatia. The results showed that all analysed samples belong to P. ripartii. Additionally, all analysed samples from different Croatian localities (Mt. Mala Kapela, Mt. Lička Plješivica, Mt. Poštak, Mt. Troglav and Mt. Kamešnica) together with previously published population of P. ripartii from Mt. Mosor belong to the same Eurasian clade of P. ripartii.
Keywords
Polyommatus (Agrodiaetus) ripartii; misidentification; Lycaenidae; Lepidoptera; COI; DNA barcoding
Hrčak ID:
160187
URI
Publication date:
30.6.2016.
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