Biochemia Medica, Vol. 26 No. 2, 2016.
Review article
https://doi.org/10.11613/BM.2016.033
Concurrent acquired inhibitors to factor VIII and IX, a laboratory artifact: a case report.
Saša Anžej Doma
; Department of Hematology, University Medical Centre Ljubljana, Slovenia
Andreas Hillarp
; Department of Clinical Chemistry and Transfusion Medicine, Halland Hospital, Halmstad, Sweden
Tadej Pajič
; Department of Hematology, University Medical Centre Ljubljana, Slovenia
Dušan Andoljšek
; Department of Hematology, University Medical Centre Ljubljana, Slovenia
Peter Černelč
; Department of Hematology, University Medical Centre Ljubljana, Slovenia
Irena Preložnik Zupan
; Department of Hematology, University Medical Centre Ljubljana, Slovenia
Abstract
Acquired inhibitors to coagulation factors other than factor VIII are extremely rare. We describe a case of a 59-year-old woman with abnormal bleeding, diagnosed with concurrent inhibitor antibodies to factor VIII and IX by Bethesda testing. We demonstrate that anti-FVIII antibodies of a very high titre are capable of disturbing the aPTT-based Bethesda assay, resulting in falsely-positive antibodies to factor IX. The case also illustrates the usefulness of the immunological assay (ELISA) in complementing the inhibitor diagnosis.
Keywords
inhibitor; autoantibody; blood coagulation factor; enzyme-linked immunosorbent assay
Hrčak ID:
161711
URI
Publication date:
15.6.2016.
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