Original scientific paper
A microcolorimetric determination of creatinine in serum or plasma by the Jaffe's reaction
V. Habazin-Novak
; Institute for Medical Research and Occupational Health, Zagreb, Croatia
R. Pleština
; Institute for Medical Research and Occupational Health, Zagreb, Croatia
Abstract
A micro procedure requiring 200 µl of sera for the analysis of creatinine concentration in a large humber of samples collected in a mass campaign during the screening of the people for Balkan endemic nephropathy has been developed. The reliability of the micro procedure was proved by examination of the precision 1.79 mg/100 ml (SD = 0.12), the exactitude was from 0.5 to 6.0 mg/100 ml. The experiments on rat's plasma showed a very high correlation between the results or the macro and micro procedure (r = 0.92). An average creatinin concentration found in 82 healthy men was 1.24 mg/100 ml (SD = 0.18), in 15 healthy women 1.08 mg/100 ml (SD = 0.20) and in 51 healthy children 0.79 mg/100 ml (SD = 0.27) of serum. Creatinine concentration did not considerably change when scrum samples were stored at 4 °C for 48 hours or at -18 °C for one year.
Keywords
Hrčak ID:
161811
URI
Publication date:
14.6.1979.
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