Original scientific paper
INVESTIGATION ON THE EFFICIENCY OF THE SUPEROVULATION AND SUPEROVULATORY RESPONSE AND THE NUMBER OF RECOVERED EMBRYOS AND THE RESULTS OF THEIR TRANSFER IN COWS AND HEIFFERS
Zdenko Makek
; Veterinarski fakultet, Zagreb, Republika Hrvatska
Miroslav Herak
; Veterinarski fakultet, Zagreb, Republika Hrvatska
Marijan Cergolj
; Veterinarski fakultet, Zagreb, Republika Hrvatska
Damir Rudan
; Centar za reprodukciju u stočarstvu Hrvatske, Zagreb, Republika Hrvatska
Melita Herak
; Veterinarski fakultet Zagreb, Zagreb, Republika Hrvatska
Iva Barac-Getz
; Veterinarski fakultet, Zagreb, Republika Hrvatska
Tomislav Vendl
; PIK Vrbovec, Govedarska farma Poljanski Lug, Republika Hrvatska
Zvonko Tuček
; "Zdenka" farma muznih krava, Veliki Zdenci, Republika Hrvatska
Ivica Cerovečki
; PIK Vrbovec, Govedarska farma Poljanski Lug, Republika Hrvatska
Krešimir Mihatović
; Centar za reprodukciju u stočarstvu Hrvatske, Zagreb, Republika Hrvatska
Tomislav Dobranić
; Veterinarski fakultet, Zagreb, Republika Hrvatska
Abstract
Investigations on the efficiency of the superovulation and superovulatory response and the number of recovered embryos and also the transfer results of fresh and cryopreserved/thawed bovine embryos were performed on 35 females. Superovulation was induced using PMSG (FolligonR) or FSH (FolltropinR) preparations in 27 females. The results of the superovulation and superovulatory respons on day 7 after induced ovulation and A.l. were followed up by rectal and ultrasonographic examinalion of the ovaries (ALOHA ECHO CAMERA SSD-210, DX ll with 5 MHz transrectal transducer) and 154 (ˉx = 5.70 ±0.69 ) corpora lutea were established. In 23 females a total of 15 embryos were received and 10 embryos were classified as transferable and then one embryo was instantly transferred into the selected recipient. The other 9 embryos were cryopreserved using freezing machine (LE NICOOBAC MS 21). After some time embryos were thawed and following classification 7 were transferred into 7 synchronized recipients. In 3 recipinets estrus reappeared after 25 days, in one after 30 days, and in 3 recipients 35 days after the previous estrus, whereas the pregrancy was ultrasonically established in one recipient on the 34th day.
Keywords
Hrčak ID:
163711
URI
Publication date:
22.1.1995.
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