Original scientific paper
Stable and Unstable Chromosome Aberrations Measured after Occupational Exposure to Ionizing Radiation and Ultrasound
Aleksandra Fučić
Davor Želježić
Vilena Kašuba
Nevenka Kopjar
Ružica Rozgaj
Ružica Lasan
August Mijić
Vlasta Hitrec
Joe Nathan Lucas
Abstract
Aim To evaluate chromosome aberration and fluorescent in situ
hybridization (FISH) assays as a method to estimate of health risk,
we monitored 9 male subjects occupationally exposed to low doses
of both ionizing radiation and ultrasound during a period of over
3 years. Methods Sampling was performed at 6-month intervals during a three-year period. First we used conventional chromosomal aberrations analysis. When the aberration frequency for a particular
subject reached the background, we measured translocations in
the final sample, using fluorescence in situ hybridization. Chromosome painting probes for chromosomes 1, 2, and 4 were used
simultaneously. Results Dicentric and ring chromosomes were eliminated within a year. Translocations persisted and deviated from control values in all examinees. Translocations were detected long after unstable aberrations decreased to the background level.
Conclusion Fluorescence in situ hybridization-based translocation
detection was a reliable method for monitoring chronic
occupational clastogen exposure. Chromosome aberration assay
correlated with translocation frequency. Stable chromosomal
aberrations reflected cumulative genome damage during job exposure.
Keywords
chromosome aberration; Ultrasound
Hrčak ID:
16448
URI
Publication date:
20.6.2007.
Visits: 1.539 *