Original scientific paper

THE PROTECTIVE ROLE OF VITAMIN C AND CHITOSAN AGAINST PARAQUAT-INDUCED OXIDATIVE STRESS IN MUSCLES OF COMMON CARP Cyprinus carpio

Zeinab Sharifinasab ; Department of Aquaculture, Faculty of Natural Resources and Environment, Behbahan Khatam Al-Anbia University of Technology, Iran
Mahdi Banaee orcid.org/0000-0001-6492-2096 ; Department of Aquaculture, Faculty of Natural Resources and Environment, Behbahan Khatam Al-Anbia University of Technology, Iran
Mohammad Mohiseni ; Department of Aquaculture, Faculty of Natural Resources and Environment, Behbahan Khatam Al-Anbia University of Technology, Iran
Ahmad Noori ; Department of Aquaculture, Faculty of Marine Science and Technology, Hormozgan University, Bandar Abbas, Iran

Abstract

The purpose of this study was to examine the effects of antioxidants, including vitamin C, chitosan or a combination of both, on oxidative stress markers in muscles, as edible tissues of fish, exposed to paraquat. Fish exposed to 0.02 mg/L paraquat for 21 days were fed different diets: a normal diet, a diet containing chitosan (1000 mg/kg diet), a diet with vitamin C (1000 mg/kg diet) or both vitamin C and chitosan. Oxidative stress markers, including the activity of catalase, total antioxidant and malondialdehyde (MDA), as well as biochemical parameters including the activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine phosphokinase (CPK), lactate dehydrogenase (LDH) and acetylcholinesterase (AChE), were measured in muscles. Fish exposure to paraquat increased LDH, CPK, catalase and MDA activity significantly, while it significantly decreased AST, ALT and AChE activity and total antioxidant capacity in muscles. Administration of vitamin C, combined with chitosan, to fish exposed to paraquat was effective in regulating AChE, AST, ALT, LDH, CPK and catalase activity. A significant increase in the total antioxidant status and a significant decrease in MDA levels were observed in fish fed chitosan-vitamin C complex. In conclusion, it is suggested that combined supplementation with vitamin C and chitosan may improve the detoxification system in the muscles of fish and protect common carp from paraquat toxicity.

Keywords

Antioxidants; Chitosan; Vitamin C; Paraquat; Biomarkers; Oxidative stress; Muscle

Hrčak ID:

169512

URI

https://hrcak.srce.hr/169512

Publication date:

28.11.2016.

Article data in other languages: croatian

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