Veterinary Archives, Vol. 86 No. 6, 2016.
Short communication, Note
Plasma levels of nitric oxide and nitric oxide synthase vis à vis ovarian steroid hormones at and around estrus in cycling cows - a short communication.
Shailaza Sharma
; Department of Biochemistry, College of Veterinary Science and Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Pashuchikitsa Vigyan Vishvavidyalya evam Go Anusandhan Sansthan (DUVASU), Mathura, Uttar Pradesh, India
Rajesh Nigam
; Department of Biochemistry, College of Veterinary Science and Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Pashuchikitsa Vigyan Vishvavidyalya evam Go Anusandhan Sansthan (DUVASU), Mathura, Uttar Pradesh, India
Vijay Pandey
; Department of Biochemistry, College of Veterinary Science and Animal Husbandry, UP Pandit Deen Dayal Upadhyaya Pashuchikitsa Vigyan Vishvavidyalya evam Go Anusandhan Sansthan (DUVASU), Mathura, Uttar Pradesh, India
Sarvpreet S. Ghuman
; Department of Veterinary Gynecology and Obstetrics, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Science University (GADVASU), Ludhiana, Punjab, India
Abstract
Blood samples were collected from cycling Sahiwal cows at -2,-1, 0, +1, and +2 days of the estrus cycle and analyzed for plasma levels of Nitric oxide (NO) and Nitric Oxide Synthase (NOS) vis a vis ovarian steroidal hormones viz. estradiol-17β and progesterone. The results revealed a gradual increase in NO, NOS and Estradiol-17β levels from day -2 and they peaked at the day of estrus, followed by a steep fall up to day +2. The values of NO, NOS and estradiol-17β ranged between 7.93 to 18.92 μM/L, 0.39 to 0.95 U/L and 5.46 to 20.16 pg/mL, respectively. The levels of progesterone ranged from 0.15 to 0.71 ng/mL and showed a significant decline from day -2 to the day of estrus and then remained statistically parallel. In addition, levels of estradiol-17β exhibited a highly significant (P<0.01) association with levels of NO and NOS. The present study generated data on NO, NOS, estradiol-17β and progesterone at and around estrus in cows, which is of practical importance for clinical and experimental interpretation.
Keywords
cows; nitric oxide; nitric oxide synthase; estradiol 17β; progesterone; estrus
Hrčak ID:
170236
URI
Publication date:
22.11.2016.
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