Original scientific paper
https://doi.org/10.15177/seefor.16-12
HPLC/MS-TOF Analysis of Surface Resins from Three Poplar Clones Grown in Serbia
Branislav Trudić
; University of Novi Sad, Institute of Lowland Forestry and Environment, Antona Čehova 13d, RS-21000 Novi Sad, Serbia
Boban Anđelković
; University of Belgrade, Faculty of Chemistry, Centre for Instrumental Analysis, Studentski trg 12-16, RS-11158, Belgrade, Serbia
Saša Orlović
; University of Novi Sad, Institute of Lowland Forestry and Environment, Antona Čehova 13d, RS-21000 Novi Sad, Serbia
Vele Tešević
; University of Belgrade, Faculty of Chemistry, Centre for Instrumental Analysis, Studentski trg 12-16, RS-11158, Belgrade, Serbia
Andrej Pilipović
; University of Novi Sad, Institute of Lowland Forestry and Environment, Antona Čehova 13d, RS-21000 Novi Sad, Serbia
Mirjana Cvetković
; University of Belgrade, Institute of Chemistry, Technology and Metallurgy, Njegoševa 12, RS-11000, Belgrade, Serbia
Jovana Stanković
; University of Belgrade, Institute of Chemistry, Technology and Metallurgy, Njegoševa 12, RS-11000, Belgrade, Serbia
Abstract
Background and Purpose: Poplar clones grown in Serbia are fast growing tree species important for many different purposes in forestry and industry. In this study chemical content of the surface resins of three poplar clones grown in Serbia - M1, B229 and PE 19/66 was analyzed, aiming at their potential usage as a source of natural products important for pharmacy and chemotaxonomy.
Materials and Methods: Using HPLC/MS-TOF we gained the first information on chemical compounds which comprise of resins on terminal twigs cuttings of commonly grown poplar clones. Provided from the nursery of the Institute of Lowland Forestry and Environment (Serbia), terminal twigs cuttings with leaves of different development stage from two year old seedlings of M1 poplar clone (Populus euramericana L.), PE 19/66 clone and B229 clone (both belonging to Populus deltoides) were sampled. The washing of the surface resins from terminal twigs cuttings of every sample was done with methylene-chloride until the samples were prepared for HPLC/MS-TOF analysis.
Results: Out of 38 different compounds which were identified, M1 clone qualitatively differed for 14 compounds as compared to two other clones. Generally, the results showed that the composition of the resins consisted of different phenolic acids, phenolic esters, flavonoids and other contents.
Conclusion: These three poplar clones are potent producers of pharmacologically and chemotaxonomically potent compounds in forest ecosystems, especially M1 clone.
Keywords
phenols; poplars; resins; terminal twigs
Hrčak ID:
170308
URI
Publication date:
9.12.2016.
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