Skip to the main content

Acta Pharmaceutica, Vol. 67 No. 2, 2017.

Short communication, Note

https://doi.org/10.1515/acph-2017-0015

Porphyra-334, a mycosporine-like amino acid, attenuates UV-induced apoptosis in HaCaT cells

SUNG-SUK SUH ; Division of Polar Life Sciences, Korea Polar Research Institute, Incheon, 21990, Republic of Korea
SE KYUNG OH ; Division of Polar Life Sciences, Korea Polar Research Institute, Incheon, 21990, Republic of Korea
SUNG GU LEE ; Division of Polar Life Sciences, Korea Polar Research Institute, Incheon, 21990, Republic of Korea; Department of Polar Science, University of Science and Technology, Incheon, 21990, Republic of Korea
IL-CHAN KIM ; Division of Polar Life Sciences, Korea Polar Research Institute, Incheon, 21990, Republic of Korea; Department of Polar Science, University of Science and Technology, Incheon, 21990, Republic of Korea
SANGHEE KIM ; Division of Polar Life Sciences, Korea Polar Research Institute, Incheon, 21990, Republic of Korea; Department of Polar Science, University of Science and Technology, Incheon, 21990, Republic of Korea

Full text: english pdf 1.742 Kb

page 257-264

downloads: 737

cite

Abstract

The main aim of the current research was to study the effect of porphyra-334, one of mycosporine-like amino acids (MAAs), well known as UV-absorbing compounds, on UV-induced apoptosis in human immortalized keratinocyte (HaCaT) cells. Due to their UV-screening capacity and ability to prevent UV-induced DNA damage, MAAs have recently attracted considerable attention in both industry and research in pharmacology. Herein, human HaCaT cells were used to determine the biological activities of porphyra-334 by various in vitro assays, including proliferation, apoptosis and Western blot assays. The proliferation rate of UV-irradiated HaCaT cells was significantly decreased compared to the control group. Pretreatment with porphyra-334 markedly attenuated the inhibitory effect of UV and induced a dramatic decrease in the apoptotic rate. Expression of active caspase-3 protein was increased in response to UV irradiation, while caspase-3 levels were similar between cells treated with porphyra-334 and the non-irradiated control group. Taken together, our data suggest that porphyra-334 inhibits UV-induced apoptosis in HaCaT cells through attenuation of the caspase pathway.

Keywords

porphyra-334; apoptosis; cell proliferation; UV irradiation; HaCaT cells

Hrčak ID:

173060

URI

https://hrcak.srce.hr/173060

Publication date:

30.6.2017.

Visits: 1.633 *