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https://doi.org/10.21860/medflum2017_173387
Proteomics analysis of potential biomarkers of acute kidney injury in urine from patients receiving vancomycin
Ivona Štanfel
; Odjel za biotehnologiju, Sveučilište u Rijeci, Rijeka
Marko Klobučar
; Odjel za biotehnologiju, Sveučilište u Rijeci, Rijeka
Sandra Kraljević Pavelić
; Odjel za biotehnologiju, Sveučilište u Rijeci, Rijeka
Ana Vujaklija Brajković
; Zavod za intenzivnu medicinu, Klinika za unutarnje bolesti, KBC Zagreb, Zagreb
Mirela Sedić
; Odjel za biotehnologiju, Sveučilište u Rijeci, Rijeka
Abstract
Aim: Vancomycin is an antibiotic used to treat infections caused by Gram-positive bacteria which can induce renal injury in some patients. Since standard biomarkers of kidney injury lack sufficient sensitivity and specificity, there is an unmet need for novel biomarkers that would enable early detection of renal injury and timely cessation or modification of treatment to prevent more severe kidney injury. The goal of this paper was to compare proteomics profiles of urine samples collected from two vancomycin-treated patients suffering from pneumonia with and without clinical symptoms of drug-induced renal dysfunction as to identify novel potential protein biomarkers of vancomycin-induced kidney injury. Case report: Urine samples were collected on the third and seventh day of vancomycin therapy from patients with and without clinical signs of acute kidney injury. Samples were analysed by two-dimensional gel electrophoresis and MALDI-TOF/TOF mass spectrometry. Differences in urinary proteomes between these two patients could be observed on the third and especially on the seventh day of therapy. Two-dimensional gel electrophoresis combined with mass spectrometry confirmed previously established role of neutrophil gelatinase-associated lipocalin (NGAL) as biomarker of renal injury, and revealed the expression of keratin 1 type II cytoskeletal and retinol-binding protein 4 (RBP4) only in urine from the patient with vancomycin- induced renal dysfunction. Conclusions: Keratin 1 type II cytoskeletal and retinol-binding protein 4 could be considered novel potential biomarkers of vancomycin-induced kidney injury. Additional studies with larger patient cohorts are needed to validate these findings and investigate their potential diagnostic and clinical significance.
Keywords
acute kidney injury; biomarkers; mass spectrometry; two-dimensional gel electrophoresis; urine; vancomycin
Hrčak ID:
173387
URI
Publication date:
1.3.2017.
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