Original scientific paper

Circular Dichroism as a Tool of Investigation in the B-Z Transition of DNA

Franco Quadrifolio ; Institute of Biology, Faculty of Medicine, University of Udine, 1-33100 Udine, Italy
Sabina Cauci ; Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, 1-34127, Trieste, Italy
Luigi Emilio Xodo ; Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, 1-34127, Trieste, Italy
Giorgio Manzini ; Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, 1-34127, Trieste, Italy

Abstract

Some useful CD applications aiming to investigate the B-Z
conformational change in nucleic acids as well as their inter actions
with a ruthenium complex of potential terapeutic interest are described in this paper.
The results presented here regard:
a) the energetics of the conformational transition from the B
right-handed to the Z left-handed helix in synthetic oligodeoxynucleotides with a cytosine-guanine alternating sequence;
b) The B to Z transition for sequences containing all four
canonical bases and the role of nickel ions and sodium perchlorate
in promoting this tranformation;
c) the inter action of Ru(II)(DMSO)4CI2a. compound exhibiting
a good antitumor activity in animals, with both mononucleosides
and polynucleotides.

Keywords

Hrčak ID:

175523

URI

https://hrcak.srce.hr/175523

Publication date:

10.8.1989.

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