Original scientific paper

Resonance-Enhanced CARS Spectroscopy of Biliproteins

Siegfried Schneider ; Institut fur Physikalische und Theoretische Chemie der Technischen Universitat Munchen, Lichtenbergstrasse 4, D-8046 Garching, FR Germany
Franz Baumann ; Institut fur Physikalische und Theoretische Chemie der Technischen Universitat Munchen, Lichtenbergstrasse 4, D-8046 Garching, FR Germany
Ulrich Kluter ; Institut fur Physikalische und Theoretische Chemie der Technischen Universitat Munchen, Lichtenbergstrasse 4, D-8046 Garching, FR Germany
Peter Gege ; Institut fur Physikalische und Theoretische Chemie der Technischen Universitat Munchen, Lichtenbergstrasse 4, D-8046 Garching, FR Germany

Abstract

The theoretical and experimental aspects, which are involved
in CARS spectroscopy of polyatomic molecules in liquid solution,
are reviewed. Resonance-enhanced CARS spectra of various biliproteins (APC**,PC** and its subunits) from the cyanobacterium
Mastigocladus laminosus in different states of aggregation are
shown and discussed with respect to the information which can
be derived about the structure of the tetrapyrrole chromophores.
It is concluded that upon aggregation the chromophore properties
are changed due to chromophore-protein interaction either by
changes in conformation/configuration or by changes in the protein
environment of the chromophores (microheterogeneity). Contrary
to the widely accepted assumption, the chromophore geometry
must be different in APC and PC.

Keywords

Hrčak ID:

175932

URI

https://hrcak.srce.hr/175932

Publication date:

30.11.1988.

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