Original scientific paper

https://doi.org/10.11613/BM.2017.023

Pneumatic tube system transport does not alter platelet function in optical and whole blood aggregometry, prothrombin time, activated partial thromboplastin time, platelet count and fibrinogen in patients on anti-platelet drug therapy

Dietmar Enko ; Institute of Clinical Chemistry and Laboratory Medicine, General Hospital Steyr, Steyr, Austria
Harald Mangge ; Clinical Institute of Medical and Laboratory Diagnostics, Medical University Graz, Graz, Austria
Andreas Münch ; Department of Anesthesiology and Intensive Care Medicine, Medical University Graz, Graz, Austria
Tobias Niedrist ; Clinical Institute of Medical and Laboratory Diagnostics, Medical University Graz, Graz, Austria
Elisabeth Mahla ; Department of Anesthesiology and Intensive Care Medicine, Medical University Graz, Graz, Austria
Helfried Metzler ; Department of Anesthesiology and Intensive Care Medicine, Medical University Graz, Graz, Austria
Florian Prüller ; Research Unit “Perioperative Platelet Function”, Medical University of Graz, Graz, Austria

Abstract

Introduction: The aim of this study was to assess pneumatic tube system (PTS) alteration on platelet function by the light transmission aggregometry (LTA) and whole blood aggregometry (WBA) method, and on the results of platelet count, prothrombin time (PT), activated partial thromboplastin time (APTT), and fibrinogen.
Materials and methods: Venous blood was collected into six 4.5 mL VACUETTE® 9NC coagulation sodium citrate 3.8% tubes (Greiner Bio-One International GmbH, Kremsmünster, Austria) from 49 intensive care unit (ICU) patients on dual anti-platelet therapy and immediately hand carried to the central laboratory. Blood samples were divided into 2 Groups: Group 1 samples (N = 49) underwent PTS (4 m/s) transport from the central laboratory to the distant laboratory and back to the central laboratory, whereas Group 2 samples (N = 49) were excluded from PTS forces. In both groups, LTA and WBA stimulated with collagen, adenosine-5’-diphosphate (ADP), arachidonic acid (AA) and thrombin-receptor-activated-peptide 6 (TRAP-6) as well as platelet count, PT, APTT, and fibrinogen were performed.
Results: No statistically significant differences were observed between blood samples with (Group 1) and without (Group 2) PTS transport (P values from 0.064 – 0.968). The AA-induced LTA (bias: 68.57%) exceeded the bias acceptance limit of ? 25%.
Conclusions: Blood sample transportation with computer controlled PTS in our hospital had no statistically significant effects on platelet aggregation determined in patients with anti-platelet therapy. Although AA induced LTA showed a significant bias, the diagnostic accuracy was not influenced.

Keywords

platelets; platelet aggregation; platelet function tests; clinical laboratory services; preanalytical phase

Hrčak ID:

176499

URI

https://hrcak.srce.hr/176499

Publication date:

15.2.2017.

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