Croatica Chemica Acta, Vol. 70 No. 2, 1997.
Original scientific paper
Oxidation Induced Changes in Lipid Mobility in Lipoproteins Followed by Steady State Fluorescence Anisotropy
Jasminka Brnjas-Kraljević
; School of Medicine, University of Zagreb, Zagreb, Croatia
Greta Pifat-Mrzljak
; Ruđer Bošković Institute, Zagreb, Croatia
Gabrielle Knipping
; Institute for Medical Biochemistry, University of Graz, Graz, Austria
Gunther Jurgens
; Institute for Medical Biochemistry, University of Graz, Graz, Austria
Abstract
The oxidation induced changes in the lipid layer of the lipoprotein
particles of LDL and HDLs have been followed by measuring the
steady-state polarization anisotropy of the fluorescence probe
TMA-DPH incorporated in the lipid layer. The mobility restriction
gradually increases with oxidation in LDL and HDL2 indicating the oxidation-induced structuring of lipids, while the effect of oxidation
in HDL3 is negligible.
Keywords
Hrčak ID:
176966
URI
Publication date:
1.5.1997.
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