Original scientific paper

Evaluation and Selection of Optimal Solvent and Solvent Combinations in Thin-Iayer Chromatography of Flavonoids and of Phenolic Acids of Zizyphus jujuba MilI

Marica Medić-Šarić ; Department of Pharmaceutical Chemistry, Faculty of Pharmacy and Biochemistry, University of Zagreb, 10000 Zagreb, Croatia
Željan Maleš ; Department of Pharmacognosy, Faculty of Pharmacy and Biochemistry, University of Zagreb, 10000 Zagreb, Croatia
Gordana Stanić ; Department of Pharmacognosy, Faculty of Pharmacy and Biochemistry, University of Zagreb, 10000 Zagreb, Croatia
Slavko Šarić ; Faculty of Traffic Engineering, University of Zagreb, 10000 Zagreb, Croatia

Abstract

A series of simple mathematical techniques for the evaluation of
solvents and solvent combinations in thin-layer chromatography
have been investigated. The classification has been carried out using
the numerical taxonomy techniques and information content
derived from Shannon's equation. The methods have been applied
to an RF data set of flavonoids and phenolic acids identified in the
methanolic extract of Zizyphus jujuba MilI. The most suitable chromatographic systems for the separation and future isolation of flavonoids and phenolic acids from Zizyphus jujuba Mill. are : ethyl
acetate : formic acid : acetic acid : water, 100 : 11 : 11 : 27, and
ethyl acetate : formic acid : water, 8 : 1 : 1.

Keywords

Hrčak ID:

177144

URI

https://hrcak.srce.hr/177144

Publication date:

1.11.1996.

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