Original scientific paper

Phylogenetic analysis of the partial VP2 gene of canine parvovirus-2 from the northern region of India

Jobin Thomas ; Immunology Section, Indian Veterinary Research Institute, Izatnagar, UP, India
Mithilesh Singh ; Immunology Section, Indian Veterinary Research Institute, Izatnagar, UP, India
Tapas K. Goswami ; Immunology Section, Indian Veterinary Research Institute, Izatnagar, UP, India
Suman Verma ; Immunology Section, Indian Veterinary Research Institute, Izatnagar, UP, India

Abstract

Canine parvovirus-2 (CPV-2) infection is a serious problem causing a high rate of mortality in puppies. Despite the widespread vaccination of domestic dogs, a major impediment in the control of this deadly disease is the presence of different antigenic variants in field. Regular surveillance and constant monitoring of these variants, which might evade the host immune pressure and laboratory detection, is critically essential. Thus, the present study was aimed at understanding the molecular epidemiology of CPV-2 strains circulating in northern region of India. Polymerase Chain Reaction (PCR) positive samples were subjected to oligonucleotide sequencing and these isolates were found to be identical to CPV-2a except at positions 264, 297 and 440 amino acid residue, and thus typed as an antigenic variant of CPV-2a. The mutation at position 264 has not been reported from India before. Furthermore, global phylogenetic analysis confirmed the molecular relationship of these new CPV-2a isolates with sequences from China.

Keywords

CPV-2; polymerase chain reaction; antigenic variants

Hrčak ID:

178521

URI

https://hrcak.srce.hr/178521

Publication date:

15.1.2017.

Article data in other languages: croatian

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