Original scientific paper

Knockdown of gga-miR-142-3p in developing chicken embryo: expression profile of XPO1

Pannerselvam M. Kumar ; Department of Animal Biotechnology, Madras Veterinary College, Vepery, Chennai, India
Prabhakar A. Tembhurne ; Department of Veterinary Microbiology and Animal Biotechnology Teaching and Research Cell, Nagpur Veterinary College, Seminary Hills, Nagpur, India
Paramasivam Raja ; Department of Animal Biotechnology, Madras Veterinary College, Vepery, Chennai, India
Vijay C. Ingle ; Department of Veterinary Microbiology and Animal Biotechnology Teaching and Research Cell, Nagpur Veterinary College, Seminary Hills, Nagpur, India
Devanand R. Kalorey ; Department of Veterinary Microbiology and Animal Biotechnology Teaching and Research Cell, Nagpur Veterinary College, Seminary Hills, Nagpur, India

Abstract

Differential expression of gga-miR-142-3p microRNA of haemopoeitic origin during immune organ development and functional stages in chicken embryos creates new opportunities for understanding its pivotal role during embryonic developmental stages. To decipher the role of gga-miR-142-3p in-ovo knockdown was carried out with LNA modified anti-miR-gga-miR-142-3p via intravenous route at developmental and functional stages of the immune organs and other organs. Bioinformatic analysis of the genes targeted by gga-miRNA-142-3p revealed that the predicted gene XPO1 conserved binding sites at 3’UTR. The target gene XPO1 was evaluated as the validated target of gga-miR-142-3p by employing qPCR SYBR green based technology, which was evidenced by its increased expression in the tissues of gga-miR-142-3p knockdown chicken embryos. Histopathological alterations in the immune organs and visceral organs indicated that the systemic knockdown of gga-miR-124-3p led to over expression of the XPO1 gene during the embryonic stages, and changed the environment of the immune organs related to structural integrity, immune response, signal transduction and migration of B and T cells during the embryonic developmental stage in the chicken embryos. The results clearly indicated that these changes could alter the postnatal development and functions of these immune organs, and may lead to development of immuno-compromised chickens.

Keywords

gga-miR-142-3p knockdown; chicken embryo; XPO1 expression

Hrčak ID:

178524

URI

https://hrcak.srce.hr/178524

Publication date:

15.1.2017.

Article data in other languages: croatian

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