Original scientific paper

The effect of dilution temperature by two extenders with different specifications on boar semen quality

Vasiliki Alexiou ; Faculty of Health Sciences, School of Veterinary Medicine, Clinic of Farm Animals, Aristotle University of Thessaloniki, Thessaloniki, Greece
Athina G. Basioura ; Faculty of Health Sciences, School of Veterinary Medicine, Clinic of Farm Animals, Aristotle University of Thessaloniki, Thessaloniki, Greece
Georgios Tsousis ; Faculty of Health Sciences, School of Veterinary Medicine, Clinic of Farm Animals, Aristotle University of Thessaloniki, Thessaloniki, Greece
Eleni D. Tzika ; Faculty of Health Sciences, School of Veterinary Medicine, Clinic of Farm Animals, Aristotle University of Thessaloniki, Thessaloniki, Greece
Constantin M. Boscos ; Faculty of Health Sciences, School of Veterinary Medicine, Clinic of Farm Animals, Aristotle University of Thessaloniki, Thessaloniki, Greece
Georgios Vatzias ; Faculty of Agriculture Forestry and Natural Environment, School of Agriculture, Animal Production, Aristotle University of Thessaloniki, University Campus, Thessaloniki, Greece
Ioannis A. Tsakmakidis ; Faculty of Health Sciences, School of Veterinary Medicine, Clinic of Farm Animals, Aristotle University of Thessaloniki, Thessaloniki, Greece

Abstract

This study investigated the quality characteristics and the field fertility of boar semen after dilution with OptimIA, a common extender at 23 (n = 20, group A23) or at 30 °C (n = 20, group A30), and after dilution with OptimIA commercial extender at 23 °C (n = 20, group 23A) or with Androhep Plus, a membrane protective extender at 23 °C (n = 20, group 23B). Each sample of extended semen was stored (16-18 °C) and used for a double artificial insemination at 48 h and 72 h after its collection at the farm (n = 30 per group). The semen was assessed in the laboratory (kinetic parameters, morphology and DNA fragmentation) at collection (0 h) and at insemination hours (48 and 72 h). Most of the semen laboratory parameters deteriorated from 48 h to 72 h, regardless of dilution temperature or the use of the protective extender. However, in the special protective extender the percentages of rapid movement spermatozoa, VCL (curvilinear velocity), VAP (average path velocity) and WOB (wobble) did not differ between 48 h and 72 h. A lower farrowing rate was observed in the common extender group at 23 °C, and a lower number of live born piglets in the protective extender group compared to the other two groups. In conclusion, one step dilution of boar semen at 23 °C compared to dilution at 30 °C did not dramatically affect its in vitro quality characteristics after 72 h of storage, although field fertility was negatively influenced. Some of these negative effects can be compensated for by the use of a membrane protective extender.

Keywords

boar semen; extender; field fertility; semen quality; temperature effect

Hrčak ID:

178600

URI

https://hrcak.srce.hr/178600

Publication date:

15.3.2017.

Article data in other languages: croatian

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