Original scientific paper

Interleukin-28B polymorphism in persons with chronic hepatitis C in Croatia

Ivana Grgić ; Klinika za infektivne bolesti "Dr. Fran Mihaljević", Zagreb, Hrvatska
Lana Gorenec ; Klinika za infektivne bolesti "Dr. Fran Mihaljević", Zagreb, Hrvatska
Martina Gašpar ; Klinika za infektivne bolesti "Dr. Fran Mihaljević", Zagreb, Hrvatska
Mia Čerina ; Klinika za infektivne bolesti "Dr. Fran Mihaljević", Zagreb, Hrvatska
Ana Planinić ; Klinika za infektivne bolesti "Dr. Fran Mihaljević", Zagreb, Hrvatska
Maja Trupković ; Klinika za infektivne bolesti "Dr. Fran Mihaljević", Zagreb, Hrvatska
Adriana Vince ; Klinika za infektivne bolesti "Dr. Fran Mihaljević", Zagreb, Hrvatska
Snježana Židovec Lepej ; Klinika za infektivne bolesti "Dr. Fran Mihaljević", Zagreb, Hrvatska

Abstract

Single nucleotide polymorphism (SNP) rs12979860 in the promotor region of the gene coding for interleukin-28B (IL-28B) is associated with the natural history of hepatitis C virus (HCV) infection and response to antiviral and immunomodulatory therapy with combination of pegylated interferon alpha and ribavirin (dual therapy). Despite the fact that the predictive role IL-28B genotyping is decreasing in the era of highly successful direct acting antiviral (DAA)-based treatment, it remains to be useful as a tool for predicting a natural course of HCV infection. In resource-limited settings with restricted access to new treatment options IL-28B polymorphism typing is still a part of pre-treatment workup before admission of dual therapy. The aim of this study was to analyse the results of IL-28B genotyping (percentages of C and T homozygotes and heterozygotes) for 595 Caucasian patients with chronic hepatitis C receiving clinical care at the Department of Viral Hepatitis of the University Hospital for Infectious Diseases, Zagreb and Croatian Reference Center for Viral Hepatitis since January 2013 till December 2015. Detection of single nucleotide polymorphism (SNP) rs12979860 was determined by using a real-time PCR test. Analysis of rs12979860 SNP in the IL-28B gene showed that 335 of 595 (56.3%) patients were CT heterozygotes, followed by C homozygotes (173 of 595 patients, 29.1%) whereas T homozygotes were the least frequent (87 of 595 patients, 14.6%). The patterns of IL-28 polymorphism in Croatian patients with chronic hepatitis C is in accordance with other results for Caucasians.

Keywords

Chronic hepatitis C; interleukin 28B; sustained virological response; single nucleotide polymorphism

Hrčak ID:

182357

URI

https://hrcak.srce.hr/182357

Publication date:

31.3.2016.

Article data in other languages: croatian

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