Original scientific paper
IMMUNOMODULATORY AND GROWTH-PROMOTING POTENTIAL OF LOWCOST PROBIOTIC PRODUCT IN Penaeus monodon CULTURE SYSTEM
Manohar Navin Chandran1,
Arumugampillai Manimehalai Suganya
Grasian Immanuel
Arunachalam Palavesam
Abstract
The capability of a low-cost probiotic product was evaluated in Penaeus
monodon reared under an outdoor culture system for a period of 90 days.
The low-cost probiotic product was formulated by mixing the broth culture
of Bacillus cereus along with commercial chalk powder in 1:1 ratio. The
prepared probiotic product as a powdered supplement was incorporated
along with the shrimp diet at various concentrations (1 to 5%). The viability
of B. cereus in the experimental diet was tested once in 30 days up to 90
days during the experimentation. After 90 days of feeding experiments, the
growth performance of shrimp was determined with a maximum production
of 11.98 ± 0.367 g, SGR of 5.030 ± 0.171% and FCE of 86.63 ± 1.300% in
P. monodon fed D4 diet (4% probiotic supplement). The immunological
parameters determined at the end of the culture experiment showed
variation among diets. The total haemocyte count (273.66 ± 3.09 x 105
cells/ml), phenoloxidase activity (0.132 ± 0.007 OD) and respiratory burst
activity (0.291 ± 0.020 O.D) were high in P. monodon fed D4 diet. Similar
effects were observed for plasma protein concentration (68.00 ± 1.41 mg/
ml), lysozyme activity (0.483 ± 0.014 U/ml) and bactericidal activity (81.0
± 1.33%) in shrimp fed D4 diet. The results of the present investigation
conclude that the probiotic product at a concentration of 4% was effective
in elevating the growth and immune performances in shrimp P. monodon.
Keywords
Low-cost probiotic product; Growth; Phenoloxidase; THC; Respiratory burst
Hrčak ID:
182655
URI
Publication date:
7.6.2017.
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