Preliminary communication

https://doi.org/10.15644/asc51/2/7

Variant Purification of an Allogeneic Bone Block

Jonas Lorenz ; FORM-Lab, Department of Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Frankfurt am Main, Germany
Markus Schlee ; Private Practice, Forchheim, Goethe University, Frankfurt, Germany
Sarah Al-Maawi ; FORM-Lab, Department of Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Frankfurt am Main, Germany
Poju Chia ; FORM-Lab, Department of Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Frankfurt am Main, Germany
Robert A. Sader ; FORM-Lab, Department of Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Frankfurt am Main, Germany
Shahram Ghanaati orcid.org/0000-0002-7897-0854 ; FORM-Lab, Department of Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of the Goethe University Frankfurt, Frankfurt am Main, Germany

Abstract

Objective: This short communication reports on a histological analysis of the composition of the commercially available Maxgraft® allogeneic bone block. Materials and Methods: Based on previously published, easily applicable histological methods, blanc samples of the Maxgraft® allogeneic bone block have been decalcified, dehydrated and embedded in paraffin before histological and histochemical staining. Afterwards, the slides were evaluated for their material characteristics, such as the bone matrix structure and other components, including collagen or cells/cell remnants. Results: The
results show that this bone block exhibits a trabecular structure with lamellar sub-organization. Additionally, cellular remnants within the osteocyte lacunae and at the outer trabecular surfaces reside together with remnants of the former inter-trabecular fatty and connective tissue, i.e., collagenous structures and connective tissue cells or cell remnants. Conclusion: Consistent with a previous study on this topic, the data presented here demonstrate that some of the certified purification techniques might not allow for the production of allogeneic materials free of organic cell and tissue components.

Keywords

Transplantation, homologous; Bone Substitutes; Biocompatible Materials; Decalcification Technique; Desiccation

Hrčak ID:

183249

URI

https://hrcak.srce.hr/183249

Publication date:

20.6.2017.

Article data in other languages: croatian

Visits: 1.619 *