Original scientific paper

https://doi.org/10.11613/BM.2017.038

A nationwide multicentre study in Turkey for establishing reference intervals of haematological parameters with novel use of a panel of whole blood

Yesim Ozarda ; Department of Medical Biochemistry, Uludag University School of Medicine, Bursa, Turkey
Kiyoshi Ichihara ; Department of Laboratory Sciences, Faculty of Health Sciences, Yamaguchi University Graduate School of Medicine, Ube, Japan
Ebubekir Bakan ; Department of Medical Biochemistry, Ataturk University School of Medicine, Erzurum, Turkey
Harun Polat ; Department of Medical Biochemistry, Ataturk University School of Medicine, Erzurum, Turkey
Nurinnisa Ozturk ; Department of Medical Biochemistry, Ataturk University School of Medicine, Erzurum, Turkey
Nurcan K Baygutalp ; Department of Medical Biochemistry, Ataturk University School of Medicine, Erzurum, Turkey
Fatma Taneli ; Department of Medical Biochemistry, Celal Bayar University School of Medicine, Manisa, Turkey
Yesim Guvenc ; Department of Medical Biochemistry, Celal Bayar University School of Medicine, Manisa, Turkey
Murat Ormen ; Department of Medical Biochemistry, Dokuz Eylul University School of Medicine, Izmir, Turkey
Zubeyde Erbayraktar ; Department of Medical Biochemistry, Dokuz Eylul University School of Medicine, Izmir, Turkey
Nurten Aksoy ; Department of Medical Biochemistry, Harran University School of Medicine, Urfa, Turkey
Hatice Sezen ; Department of Medical Biochemistry, Harran University School of Medicine, Urfa, Turkey
Meltem Demir ; Department of Medical Biochemistry, Medicalpark Hospital, Antalya, Turkey
Gulcin Eskandari ; Department of Medical Biochemistry, Mersin University School of Medicine, Mersin, Turkey
Gurbuz Polat ; Department of Medical Biochemistry, Mersin University School of Medicine, Mersin, Turkey
Nuriye Mete ; Department of Medical Biochemistry, Dicle University School of Medicine, Diyarbakir, Turkey
Hatice Yuksel ; Department of Medical Biochemistry, Dicle University School of Medicine, Diyarbakir, Turkey
Husamettin Vatansev ; Department of Medical Biochemistry, Selçuk University School of Medicine, Konya, Turkey
Fatma Gun ; Department of Medical Biochemistry, Selçuk University School of Medicine, Konya, Turkey
Okhan Akin ; Department of Medical Biochemistry, Keçiören Teaching and Research Hospital, Ankara, Turkey
Ozlem Ceylan ; Department of Medical Biochemistry, Keçiören Teaching and Research Hospital, Ankara, Turkey
Tevfik Noyan ; Department of Medical Biochemistry, Ordu University School of Medicine, Ordu, Turkey
Ozgul Gozlukaya ; Department of Medical Biochemistry, Ordu University School of Medicine, Ordu, Turkey
Yuksel Aliyazicioglu ; Department of Medical Biochemistry, Karadeniz Teknik University School of Medicine, Trabzon, Turkey
Sevim Kahraman ; Department of Medical Biochemistry, Karadeniz Teknik University School of Medicine, Trabzon, Turkey
Melahat Dirican ; Department of Medical Biochemistry, Uludag University School of Medicine, Bursa, Turkey
Gul Ozlem Tuncer ; Department of Medical Biochemistry, Uludag University School of Medicine, Bursa, Turkey
Shogo Kimura ; Department of Laboratory Sciences, Faculty of Health Sciences, Yamaguchi University Graduate School of Medicine, Ube, Japan
Pinar Eker ; Department of Medical Biochemistry, Kuzey Laboratories, Fatih Sultan Mehmet Hospital, Istanbul

Abstract

IntroductionA nationwide multicentre study was conducted to establish well-defined reference intervals (RIs) of haematological parameters for the Turkish population in consideration of sources of variation in reference values (RVs). Materials and methodsK2-EDTA whole blood samples (total of 3363) were collected from 12 laboratories. Sera were also collected for measurements of iron, UIBC, TIBC, and ferritin for use in the latent abnormal values exclusion (LAVE) method. The blood samples were analysed within 2 hours in each laboratory using Cell Dyn and Ruby (Abbott), LH780 (Beckman Coulter), or XT-2000i (Sysmex). A panel of freshly prepared blood from 40 healthy volunteers was measured in common to assess any analyser-dependent bias in the measurements. The SD ratio (SDR) based on ANOVA was used to judge the need for partitioning RVs. RIs were computed by the parametric method with/without applying the LAVE method. ResultsAnalyser-dependent bias was found for basophils (Bas), MCHC, RDW and MPV from the panel test results and thus those RIs were derived for each manufacturer. RIs were determined from all volunteers’ results for WBC, neutrophils, lymphocytes, monocytes, eosinophils, MCV, MCH and platelets. Gender-specific RIs were required for RBC, haemoglobin, haematocrit, iron, UIBC and ferritin. Region-specific RIs were required for RBC, haemoglobin, haematocrit, UIBC, and TIBC. ConclusionsWith the novel use of a freshly prepared blood panel, manufacturer-specific RIs’ were derived for Bas, Bas%, MCHC, RDW and MPV. Regional differences in RIs were observed among the 7 regions of Turkey, which may be attributed to nutritional or environmental factors, including altitude.

Keywords

multicentre study; reference intervals; complete blood count; haematology; Turkey

Hrčak ID:

183387

URI

https://hrcak.srce.hr/183387

Publication date:

15.6.2017.

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