Original scientific paper
https://doi.org/10.15644/asc51/3/5
Frequency of Bacetrial Content Finding in Persistant Periapical Lesions
Joško Grgurević
; Department of Oral Surgery, Zagreb Dental Policlinic, Zagreb, Croatia
Ana Ivanišević Malčić
orcid.org/0000-0002-1380-0029
; Department of Endodontics and Restorative Dentistry, School of Dental Medicine, University of Zagreb, Zagreb, Croatia; University Clinical Hospital Center Zagreb, Dental Clinic
Arjana Tambić Andrašević
; Department of Microbiology, School of Medicine University of Zagreb, Croatia
Goranka Prpić Mehičić
; Department of Endodontics and Restorative Dentistry, School of Dental Medicine, University of Zagreb, Zagreb, Croatia
Sania Kuzmac
; Laboratory for Molecular Immunology, School of Medicine University of Zagreb, Croatia
Silvana Jukić
orcid.org/0000-0002-2305-4082
; Department of Endodontics and Restorative Dentistry, School of Dental Medicine, University of Zagreb, Zagreb, Croatia
Abstract
Objectives: To determine the percentage of persistant apical lesions positive for bacterial nucleic acids, to detect microorganisms difficult to cultivate in persistant apical lesions by PCR and relate them to endodontic failure, clinical symptoms and diabetes mellitus. Materials and methods: The samples of persistent apical lesions were collected during apicoectomy. Bacterial ubiquitous primer 16S rRNA was used to detect 16S ribosomal RNA in 36 samples. A species–specific PCR was performed with primers targeted to the bacterial 16S rRNA genes of Prevotella Nigrescens, Pseudoramibacter alactolyticus, and Propionobacterium propionicum. Results: Six samples (16.67%) were positive for bacterial ribosomal RNA. Pseudoramibacter alactolyticus was detected in three samples. Propionibacterium propionicum and Prevotella nigrescens were detected in one sample each. The prevalence
of infection of such lesions with P. intermedia, P. propionicum and P. alactolyticus is low. Conslusion: The study we conducted gave insufficient data about extraradicular infection and its connection with diabetes mellitus and clinical symptoms. Conclusions: Apical lesions persisting after endodontic treatment could harbor microorganisms other than Actinomyces and Propionicum species.
Keywords
Root Canal Preparation; Treatment Failure; periapical abscess; Bacterial RNA; 16S Ribosomal RNA; Polymerase Chain Reaction
Hrčak ID:
186809
URI
Publication date:
26.9.2017.
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