Original scientific paper
MORPHOMETRIC VARIATIONS BETWEEN TRIPLOID AND DIPLOID Clarias gariepinus (Burchell, 1822)
Jalil Normala
Azizul Alim Mohd
Munafi Ambok Bolong Abol
Asma Ariffin Nur
Waiho Khor
T. Victor Okomoda
Md Sheriff Shahreza
Abstract
Several scientific methods have been described in the identification of
triploid fish. However, many of these methods are not applicable for routine
management purposes due to their complexity and cost. In this study, the
possibility of using morphological variation as a least cost and less complex
method of distinguishing triploid and diploid African catfish Clarias
gariepinus (Burchell, 1822) was examined. Triploid catfish were produced
by cold shock of fertilized eggs in 5°C for 20 mins (at approximately 3
mins after fertilization). The fish were incubated, hatched and raised for
3 months. Ploidy levels of the fish were then ascertained by observing
the erythrocyte shape. Triploid erythrocyte was ellipsoidal in shape while
diploid was round. Morphological characterization was then carried out on
100 samples each of triploid and diploid African catfish. Although significant
differences were observed in many parameters, the principal morphometric
difference between triploid and diploid African catfish could not be clearly
distinguished. It was therefore concluded that morphological characteristics
is not ideal for discriminating triploids and diploids of African catfish. The
used of erythrocyte characteristics still remains the cheapest and relatively
effective method for triploid and diploid determination in African catfish.
Keywords
African catfish; Ploidy levels; Chromosome number; Cold shock; Erythrocyte shape
Hrčak ID:
186842
URI
Publication date:
28.9.2017.
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