Original scientific paper
https://doi.org/10.15255/CABEQ.2016.898
Strategies for Automated Control of the Bioproduction of Mcl-PHA Biopolymers
P. Hrnčiřík
orcid.org/0000-0002-6533-4541
; Department of Computing and Control Engineering, University of Chemistry and Technology, Prague, Technická 5, 166 28 Praha 6, Czech Republic
J. Náhlík
; Department of Computing and Control Engineering, University of Chemistry and Technology, Prague, Technická 5, 166 28 Praha 6, Czech Republic
J. Mareš
; Department of Computing and Control Engineering, University of Chemistry and Technology, Prague, Technická 5, 166 28 Praha 6, Czech Republic
Abstract
Medium-chain-length polyhydroxyalkanoates (mcl-PHAs) are polyesters synthesized by numerous bacteria as storage material. Despite being promising candidates for biodegradable materials of industrial interest and environmental value, their usage is still
rather limited because of high production costs. One of the areas with considerable potential for further improvements is control of the production process. This paper deals with the experimental work related to the design of control strategies for mcl-PHA biopolymer
production process (Pseudomonas putida KT2442 fed-batch cultivations). For this bioprocess, a set of five control strategies (two main and three auxiliary strategies) have been proposed, together with the proper sequence of their switching during the fedbatch
part of the production process. The application of these strategies with octanoic acid as a sole carbon source resulted in intracellular PHA content (max. mass fraction 65 % of mcl-PHA in cell dry mass (g g–1) and PHA productivity (max. 0.89 g L–1 h–1) comparable to the best results reported in the literature for this type of strain and carbon
substrate.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Keywords
fermentation; process control; biopolymer production; fed-batch cultivation
Hrčak ID:
187133
URI
Publication date:
4.10.2017.
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