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Chemical and Biochemical Engineering Quarterly, Vol. 31 No. 3, 2017.

Original scientific paper

https://doi.org/10.15255/CABEQ.2016.833

Study on the Properties of Immobilized Biocatalysts with Lipase Activity Produced by Yarrowia lipolytica in Batch Culture

I. A. Stolarzewicz ; Department of Chemistry, Faculty of Food Sciences, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-787, Warsaw, Poland
P. Zaborniak ; Interfaculty Department of Biotechnology, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-787, Warsaw, Poland
A. U. Fabiszewska orcid id orcid.org/0000-0002-4563-6649 ; Department of Chemistry, Faculty of Food Sciences, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-787, Warsaw, Poland
E. Białecka-Florjańczyk ; Department of Chemistry, Faculty of Food Sciences, Warsaw University of Life Sciences, Nowoursynowska Street 159, 02-787, Warsaw, Poland

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Abstract

Three kinds of matrices (calcium alginate, gelatin, and PVA) were employed as supports to immobilize lipases from Y. lipolytica KKP 379 via physical adsorption. The stability of biocatalysts (free and immobilized) was evaluated by measuring the enzyme activity before and after treatment with the method based on the hydrolysis of p-nitrophenyl laurate. Two fractions of enzymes were immobilized: cell-bound (yeast biomass) and extracellular (supernatant). The yield of immobilization and catalytic properties of immobilized lipases were investigated. Satisfactory results for lipolytic activity and biocatalyst
stability were obtained for cell-bound enzymes immobilized in alginate (0.38 U g–1 d.m.) and crosslinked gelatin (0.18 U g–1 d.m.). Immobilization of the supernatant was successful only on the alginate (0.026 U g–1 d.m.). After lyophilization, no significant difference was noticed between treated and untreated biocatalysts. Lyophilized catalysts were successfully immobilized in all three matrices, but the process reduced their lipolytic activity probably due to an insufficient amount of water in the reaction solution.







This work is licensed under a Creative Commons Attribution 4.0 International License.

Keywords

lipase; immobilization; Yarrowia lipolytica; alginate; gelatin; polyvinyl alcohol

Hrčak ID:

187135

URI

https://hrcak.srce.hr/187135

Publication date:

4.10.2017.

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