Original scientific paper

https://doi.org/10.11613/BM.2017.030706

The local clinical validation of a new lithium heparin tube with a barrier: BD Vacutainer® Barricor LH Plasma tube

Fatma Demet Arslan ; Department of Medical Biochemistry, University of Health Sciences, Tepecik Education and Research Hospital, Izmir, Turkey
Inanc Karakoyun ; Department of Medical Biochemistry, University of Health Sciences, Tepecik Education and Research Hospital, Izmir, Turkey
Banu Isbilen Basok ; Department of Medical Biochemistry, University of Health Sciences, Tepecik Education and Research Hospital, Izmir, Turkey
Merve Zeytinli Aksit ; Department of Medical Biochemistry, University of Health Sciences, Tepecik Education and Research Hospital, Izmir, Turkey
Anil Baysoy ; Department of Medical Biochemistry, University of Health Sciences, Tepecik Education and Research Hospital, Izmir, Turkey
Yasemin Kilic Ozturk ; Palliative Care Unit, University of Health Sciences, Tepecik Education and Research Hospital, Izmir, Turkey
Yusuf Adnan Guclu ; Palliative Care Unit, University of Health Sciences, Tepecik Education and Research Hospital, Izmir, Turkey
Can Duman ; Department of Medical Biochemistry, University of Health Sciences, Tepecik Education and Research Hospital, Izmir, Turkey

Abstract

Introduction: Although serum-providing blood tubes with a barrier are still widely used due to their significant advantages, the use of blood tubes
with a barrier to provide plasma is becoming widespread. We compared 22 analytes in a BD Vacutainer® Barricor LH Plasma tube for local clinical
validation of this new lithium heparin tube with a barrier.
Materials and methods: Samples from 44 volunteers were collected in different tubes (Becton Dickinson and Company): Z tube without additive
(reference), clot-activator tube with gel (SST), lithium heparin tube without gel (LiH), and lithium heparin tube with barrier (Barricor). Analyte
concentrations in different tubes were compared with the reference tube. All tubes were also evaluated according to additional testing (different
centrifugation durations, blood-sampling techniques and individual differences).
Results: Aspartate aminotransferase (AST), glucose (Glc), potassium (K), lactate dehydrogenase (LD), sodium (Na), and total protein (TP) had a
significant bias in Barricor (9.19%, - 3.24%, - 4.88%, 21.60%, - 0.40%, 5.03%, respectively) relative to the reference tube. There was no statistical
difference between different centrifugation durations and individual differences for AST, K and LD in LiH and/or Barricor (P > 0.05). There was a significant
bias for LD between LiH and Barricor in terms of blood-sampling techniques (21.2% and 12.4%, respectively).
Conclusions: Recently, the use of plasma has become prominent due to some of its advantages. In this study, plasma AST, K, LD, Glc and TP levels in
Barricor were clinically different in comparison to serum. The results of additional tests showed that higher levels of LD in Barricor did not result from
haemolysis, and they might be related to other factors including number of platelets, cellular fragility, or functional environment.

Keywords

serum; plasma; blood analysis; blood specimen collection; evaluation

Hrčak ID:

187589

URI

https://hrcak.srce.hr/187589

Publication date:

15.10.2017.

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