Short communication, Note

https://doi.org/10.11613/BM.2017.030901

The new Greiner FC-Mix tubes equal the old Terumo ones and are useful as glucose stabilizer after prolonged storage of samples

Graziella Bonetti ; Clinical Chemistry Laboratory, Azienda Ospedaliera Spedali Civili di Brescia, Brescia, Italy
Mariarosa Carta ; Clinical Chemistry and Haematology Laboratory, St. Bortolo Hospital, Vicenza, Italy

Abstract

Introduction: The aim of our study is to compare new Greiner tubes containing granulated citrate buffer with the Terumo ones and to verify if they
are suitable for glucose stabilization after prolonged storage.
Materials and methods: In Study 1, blood was collected in two Terumo and two Greiner tubes from 40 healthy volunteers. Samples were stored at
room temperature (RT) for 1 and 2 hours, respectively. Comparison was made by Deming regression. In Study 2, glucose was measured in a reference
tube (N = 50), according to the ADA-NACB guidelines and in aliquots of Greiner samples maintained un-centrifuged at RT for 1, 2, 4 (N = 50) and 24,
48, 72 hours (N = 35).
Results: There were insignificant mixed biases between the Terumo and Greiner tubes. Compared to reference (5.3 mmol/L), glucose concentration
in the new tubes was 5.4 (P < 0.05), 5.4 (P < 0.05), 5.3 (P = 0.265), 5.2 (P = 0.156), 5.3 (P < 0.05) and 5.2 (P < 0.05) mmol/L after 1, 2, 4, 24, 48 and
72 hours at RT, respectively. There was no biological difference between any of the time points up to 48 h (bias < ± 1.95%).
Conclusions: The study shows that the new tubes perform equally well as the Terumo ones and ensure glucose stabilization up to 48 h as well as
permit to create a link between the previous studies demonstrating the clinical utility of granulated citrate buffer and the future ones.

Keywords

diabetes; glucose; acidification, pre-analytical phase

Hrčak ID:

187595

URI

https://hrcak.srce.hr/187595

Publication date:

15.10.2017.

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