Original scientific paper

https://doi.org/10.24099/vet.arhiv.160719a

Occurrence of extended-spectrum cephalosporinase producing Escherichia coli in kuroiler birds

Pratik Ghosh ; Department of Veterinary Microbiology, West Bengal University of Animal and Fishery Sciences, Sarani, Belgachia, Kolkata, West Bengal, India
Achintya Mahanti ; Department of Veterinary Microbiology, West Bengal University of Animal and Fishery Sciences, Sarani, Belgachia, Kolkata, West Bengal, India
Indranil Samanta orcid.org/0000-0003-1189-3994 ; Department of Veterinary Microbiology, West Bengal University of Animal and Fishery Sciences, Sarani, Belgachia, Kolkata, West Bengal, India
Siddhartha N. Joardar ; Department of Veterinary Microbiology, West Bengal University of Animal and Fishery Sciences, Sarani, Belgachia, Kolkata, West Bengal, India
Kunal Batabyal ; Department of Veterinary Microbiology, West Bengal University of Animal and Fishery Sciences, Sarani, Belgachia, Kolkata, West Bengal, India
Samir Dey ; Department of Veterinary Microbiology, West Bengal University of Animal and Fishery Sciences, Sarani, Belgachia, Kolkata, West Bengal, India
Subhash Taraphder ; Department of Animal Genetics and Breeding, West Bengal University of Animal and Fishery Sciences, Sarani, Belgachia, Kolkata, West Bengal, India
Devi P. Isore ; Department of Veterinary Microbiology, West Bengal University of Animal and Fishery Sciences, Sarani, Belgachia, Kolkata, West Bengal, India

Abstract

The present study was undertaken to detect the incidence of extended-spectrum-cephalosporinase gene possessing-Escherichia coli, its co-resistance pattern against other antimicrobials, and the clonal relationship of the isolates in healthy kuroiler birds. A total of 80 cloacal swabs from kuroilers were collected randomly from West Bengal, India. The use of costly antimicrobials (cephalosporins) was not practiced by farmers. Escherichia coli was isolated and identified by standard biochemical tests and 16SrRNA-PCR. All the E. coli isolates, including controls, were subjected to PCR for detection of blaCTX-M, blaTEM, blaSHV, and blaCMY-2 genes. By comparing the RAPD-banding pattern, the phylogenetic relationship among the isolates was established. All the isolates were tested for phenotypical resistance against other antibiotics. In total, 60 isolates were identified as E. coli from the kuroilers studied (n = 80). Among them, 12 (20%) isolates possessed one of the studied extended-spectrum cephalosporinase genes. Among the studied genes, blaTEM and blaSHV were detected in 6 (10%) and 12 (20%) E. coli isolates, respectively. None of the E. coli isolates possessed blaCTX-M and blaCMY-2. In phylogenetic analysis, the strains isolated from same localities with similar genetic profile were grouped into the same cluster. Resistance of extended-spectrum cephalosporinase gene possessing E. coli isolates was observed most frequently against ampicillin/cloxacillin, co-trimoxazole, amoxyclav, piperacillin, ceftriaxone, and tetracycline. Kuroiler birds with no cephalosporin usage profile may act as a reservoir of extended-spectrum cephalosporinase gene possessing E. coli. This is the first systematic study in kuroilers, to raise the awareness of consumers regarding the possibility of transmission of antimicrobial resistant E. coli from them.

Keywords

E. coli; extended-spectrum cephalosporinase; India; kuroiler

Hrčak ID:

189400

URI

https://hrcak.srce.hr/189400

Publication date:

9.11.2017.

Article data in other languages: croatian

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