Original scientific paper
Antibacterial Effects of Endodontic Dressings on Enterococcus Faecalis in Human Root Dentine
Wilma Brugger
Vera Hofer
Peter Städtler
Abstract
The aim of this in vitro study was to assess antimicrobial activity of calcium hydroxide (Calasept) and chlorhexidine (CHX) in various concentrations with respect to Enterococcus faecalis in human root dentine up to 100 μm.
Material and Methods: Forty-eight human root canals were enlarged to standard size (ISO 40) and inoculated with Enterococcus faecalis for 21 days. After inoculation the canals were medicated with one of the following: 2 %, 1 % and 0.2 % chlorhexidin gel, Chlorhexidine
releasing gutta-percha points (active point), calcium hydroxide and aqua distillate (aqua dest.). Aqua dest. served as control medium. At the end of a disinfection period of one week dentine samples were collected with reamer and H-file (ISO 45 and 50) and put onto Columbia agar plates.
Results: Bacterial growth was assessed by counting the colony forming units (CFU) after 24 hours and 48 hours of incubation. CHX gels could penetrate dentine up to 100 μm. Two % CHX gel is slightly stronger than 1 % CHX gel (p-value 0.0925) only in the peripheral dentine sample after 48 hours of incubation, and 0.2 % CHX gel had less effects on E. faecalis than 2 % CHX (p-value 0.0191). In the central dentine sample no difference between the CHX gels could be observed. In general, CHX gels were more effective than the other medicaments tested. No significant difference between Ca(OH)2 and Aqua dest. could be observed.
Conclusion: For effective elimination of E. faecalis, especially in deeper dentine-layers, CHX concentrations of at least 1 % are necessary. An intracanal, interappointment dressing of 2 % CHX may have the potential to eliminate E. faecalis in vivo.
Keywords
Endodontics; Enterococcus faecalis; Dentine, Secondary; Anti Infective Agents, Local
Hrčak ID:
18215
URI
Publication date:
6.12.2007.
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