Professional paper

https://doi.org/10.24099/vet.arhiv.160924

Molecular characterization of extensively drug resistant (XDR), extended spectrum beta-lactamases (ESBL) and New Delhi Metallo beta-lactamase-1 (blaNDM1) producing Escherichia coli isolated from a male dog - a case report

Bommenahalli S. Pruthvishree ; Division of Epidemiology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India
Obli R. Vinodh Kumar ; Division of Epidemiology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India
Mani Sivakumar ; Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India
Shikha Tamta ; Division of Epidemiology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India
Ramanjeneya Sunitha ; Division of Veterinary Public Health, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India
Dharmendra K. Sinha ; Division of Epidemiology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India
Bhoj R. Singh ; Division of Epidemiology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India

Abstract

In this article, we report the molecular characterization of extensively drug resistant (XDR), extended spectrum, class C beta-lactamases and NDM-1 carbapenemase producing E. coli, isolated from the scrotal fluid of a 3-year-old male dog. In an antibiotic susceptibility test the E. coli isolate was susceptible only to tigecycline and resistant to all clinically applicable antibiotics tested in the study. The minimum inhibitory concentration (MIC) of meropenem, cefotaxime and cefepime was 256, 128 and 64 µg/mL, respectively. On genotypic screening by PCR, the isolate was positive for blaNDM, blaCTX-M, blaAmpC, blaTEM and sul1 genes. The isolate was a ESBL, AmpC and metalo beta-lactamase producer. On molecular pathotyping, the isolate harbored the Shiga toxin producing gene (Stx2). The extensively drug resistant, carbapenem resistant and ESBL producing E. coli constitutes a major public health concern, since there is a great chance of dissemination of resistance genes to humans due to the close association of humans and companion animals. To the best of our knowledge, this is the first report of blaNDM1 isolated from a dog in India.

Keywords

dog; NDM; ESBL; carbapenemase; beta-lactamase

Hrčak ID:

192716

URI

https://hrcak.srce.hr/192716

Publication date:

22.1.2018.

Article data in other languages: croatian

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