Original scientific paper

DISTRIBUTION OF THE MAIN ABO BLOOD GROUP ALLELES IN CROATIAN POPULATION

IRENA JUKIĆ orcid.org/0000-0003-2778-8400 ; Croatian Institute of Transfusion Medicine, Zagreb, Croatia
JASNA BINGULAC-POPOVIĆ orcid.org/0000-0002-9449-3154 ; Croatian Institute of Transfusion Medicine, Zagreb, Croatia
MARINA SAMARDŽIJA ; University of Osijek, School of Medicine, Osijek, Croatia
MARINA LAMPALO orcid.org/0000-0001-9264-6922 ; Klinički bolnički centar Zagreb, Klinika za plućne bolesti Jordanovac, Zagreb, Hrvatska
ANA HEĆIMOVIĆ ; Croatian Institute of Transfusion Medicine, Zagreb, Croatia
VESNA ĐOGIĆ orcid.org/0000-0002-6025-4649 ; Croatian Institute of Transfusion Medicine, Zagreb, Croatia
MAJA STRAUSS PATKO ; Croatian Institute of Transfusion Medicine, Zagreb, Croatia

Abstract

The frequencies of gene polymorphisms of blood groups serve as markers for populations and races. Distribution of ABO blood groups varies among populations and subpopulations around the world. The aim of the study was to determine distribution of the 5 main alleles of ABO system among Croatian blood donors and compare them with other populations. Material and Methods: The study included 303 samples of healthy unrelated volunteer blood donors, 123 female and 180 male, aged 18-65 years, as a representative sample population of Croatia. After isolation of genomic DNA using commercial columns
(QIAamp DNA Blood Mini Kit, Qiagen, Germany) or on the Magna Pure Compact (Roche Diagnostics Corp., USA) device, ABO genotypes were determined by the PCR-SSP method. Results: Twelve of 15 ABO genotypes were identifi ed. The most common was O1O1 (37.30%), followed by O1A1 (27.00%), O1B (15.80%), A1B (4.30%), A1A1 (4.00%), O1A2 (3.60%), O1O2 (2.60%), A1A2 (1.70%), O2A1 (1.70%), A2B (1.00%), BB (0.70%), and O2O2 (0.30%). Three rare ABO genotypes, A2A2, O2A2, and O2B, were not identifi ed. The calculated allele frequencies of the fi ve main alleles were as follows: O1, 0.620; O2, 0,025; A1, 0.21; A2, 0,035; and B, 0.11. In the Croatian population, O1 was found to be the most common allele, followed by A1 and B, while O2 allele was the least prevalent one. Conclusion: The distribution of alleles in Croatia is comparable to other European nations. According to the frequency of B allele, the Croatian population is comparable to Eastern Europe, probably due to migration of the population in the past. Results of ABO blood group genotyping have fundamental importance for research of the ABO system as a genetic risk factor for some diseases, as well as for anthropologic testing.

Keywords

main ABO blood group allele; ABO blood groups; voluntary blood donors

Hrčak ID:

193699

URI

https://hrcak.srce.hr/193699

Publication date:

15.2.2018.

Article data in other languages: croatian

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