Conference paper

Circular Dichroism of Partially Purified Cytochrome P450 from Rabbit Liver Microsomes

K. Ruckpaul ; Department of Biocatalysis, Central Institute of Molecular Biology, Academy of Sciences .of GDR, 1115 Berlin, GDR
H. Rein ; Department of Biocatalysis, Central Institute of Molecular Biology, Academy of Sciences .of GDR, 1115 Berlin, GDR
G.-R. Janig ; Department of Biocatalysis, Central Institute of Molecular Biology, Academy of Sciences .of GDR, 1115 Berlin, GDR
W. Winkler ; Department of Biocatalysis, Central Institute of Molecular Biology, Academy of Sciences .of GDR, 1115 Berlin, GDR
O. Ristau ; Department of Biocatalysis, Central Institute of Molecular Biology, Academy of Sciences .of GDR, 1115 Berlin, GDR

Abstract

The heme-related circular dichroic bands of solubilized cytochrome
P450 from rabbit liver microsomes and some of its liganded
derivatives were measured in the Soret region. All P450 derivatives
exhibit negative circular dichroic bands in the region of the Soret
absorption. The wavelengths of the dichroic bands and their ellipticities
vary with ligand substitution and the oxidation state of the
iron. The results are compared with CD-data from other hemoproteins
and discussed with respect to stereochemical conclusions
concerning the geometry and the physicochemical character of the
vicinity of the heme group with regard to results obtained from
other studies.

Keywords

Hrčak ID:

196373

URI

https://hrcak.srce.hr/196373

Publication date:

30.3.1977.

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