Conference paper

Characterization of the Active Site of Acetylcholinesterases by Application of Sterically Modified Acetylcholine Homologues

W. H. Hopff ; Pharmakologisches Institut der Universitat Zurich, Gloriastrasse 32, CH 8006 Zurich, Schweiz
G. Riggio ; Pharmakologisches Institut der Universitat Zurich, Gloriastrasse 32, CH 8006 Zurich, Schweiz
A. Hofmann ; Pharmakologisches Institut der Universitat Zurich, Gloriastrasse 32, CH 8006 Zurich, Schweiz
P. G. Waser ; Pharmakologisches Institut der Universitat Zurich, Gloriastrasse 32, CH 8006 Zurich, Schweiz

Abstract

Our aim was to find steric limitations for the model of the
active site of acetylcholinesterase and cho1inesterase. For thi we used homologues of acetylcholine with hydrocarbon chains
of increasing size at the cationic head. Catalysis of hydrolysis
ceased for both enzymes, when the methyl groups of the cationic
head of acetylcholine were substituted by three n-propyl groups.
With data already documented in the literature and our additional
experiments, under the same conditions, we were able to present
models of the active sites for both enzymes.

Keywords

Hrčak ID:

196598

URI

https://hrcak.srce.hr/196598

Publication date:

3.12.1975.

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