Original scientific paper

Acid Proteinases from Calf Lymph Nodes

Tamara Turnšek ; Department of Biochemistry, »Jozef Stefan« Institute and Department of Biology, University of Ljubljana, 61000 Ljubljana, Slovenia, Yugoslavia
I. Kregar ; Department of Biochemistry, »Jozef Stefan« Institute and Department of Biology, University of Ljubljana, 61000 Ljubljana, Slovenia, Yugoslavia
D. Lebez ; Department of Biochemistry, »Jozef Stefan« Institute and Department of Biology, University of Ljubljana, 61000 Ljubljana, Slovenia, Yugoslavia

Abstract

Acid proteinases were isolated from calf lymph nodes using
acid extraction, ammonium sulphate and acetone precipitation,
followed by ion exchange chromatography on CM-cellulose and
_ gel chromatography on Sephadex G-100. Cathepsin D (E. C. 3.4.23.5)
is present in lymph nodes. It has the molecular weight of 39 000
as determined by gel filtration on Sephadex G-100. Cathepsins
Bl and B2 (E. C. 3.4.22.1) were also isolated. Molecular weights
of 22 000 and 51 000 were determined for cathepsins Bl and B2,
respectively. Calf lymph nodes contain also another proteinase
which degrades haemoglobin at an optimum at pH = 3.0. This
proteinase has a molecular weight of 14 000 as was determined
by gel filtration. Its activity is not inhibited with 0,25 ~tM pepstatin
which inhibits 903/o of the cathepsin D activity.

Keywords

Hrčak ID:

196695

URI

https://hrcak.srce.hr/196695

Publication date:

28.5.1975.

Visits: 735 *